How do molecules organize into living systems?
We now have a near complete parts list of all the molecules constituting cells. However, we still only poorly understand how all these tiny molecules self-organize into much larger organelles, cells, and organisms. Our group aims to elucidate principles underlying this organization. Specifically, we study how the proteome partitions between nucleus and cytoplasm. We aim to decipher the underlying molecular mechanisms, and ask how different nuclear composition affects biological function. To address these questions we employ and develop mass-spectrometry based proteomics and combine this technology with computational, biochemical, and imaging approaches. Our main research models are human tissue culture cells and the eggs, cell-free extracts, and embryos of the frog Xenopus laevis.
Wühr M, Güttler T, Peshkin L, McAlister GC, Sonnett M, Ishihara K, et al. The Nuclear Proteome of a Vertebrate. Curr Biol. 2015; 25(20):2663-71.
Peshkin L, Wühr M, Pearl E, Haas W, Freeman RM, Gerhart JC, et al. On the Relationship of Protein and mRNA Dynamics in Vertebrate Embryonic Development. Dev Cell. 2015; 35(3):383-94.
Wühr M, Freeman RM, Presler M, Horb ME, Peshkin L, Gygi SP, et al. Deep proteomics of the Xenopus laevis egg using an mRNA-derived reference database. Curr Biol. 2014; 24(13):1467-75.
McAlister GC, Nusinow DP, Jedrychowski MP, Wühr M, Huttlin EL, Erickson BK, et al. MultiNotch MS3 enables accurate, sensitive, and multiplexed detection of differential expression across cancer cell line proteomes. Anal Chem. 2014; 86(14):7150-8.
Wühr M, Haas W, McAlister GC, Peshkin L, Rad R, Kirschner MW, et al. Accurate multiplexed proteomics at the MS2 level using the complement reporter ion cluster. Anal Chem. 2012; 84(21):9214-21.
Wühr M, Tan ES, Parker SK, H Detrich W, Mitchison TJ. A model for cleavage plane determination in early amphibian and fish embryos. Curr Biol. 2010; 20(22):2040-5.
Wühr M, Chen Y, Dumont S, Groen AC, Needleman DJ, Salic A, et al.. Evidence for an upper limit to mitotic spindle length. Curr Biol. 2008; 18(16):1256-61.