Alice Ting

School of Medicine
Stanford University
Thursday, Feb. 6, 2020
4:30pm
Edward C Taylor Auditorium, Frick B02
Host
Ralph Kleiner
Add to Calendar2020-02-06 16:30:002020-02-06 16:30:00Ralph KleinerEdward C Taylor Auditorium, Frick B0215YYYY-MM-DD

Optogenetic and chemogenetic technologies for probing molecular and cellular interactions

Spatial compartmentation underlies all cellular signaling, but existing methods to study the subcellular organization of endogenous proteins and RNA - by imaging and biochemical fractionation for example - have important limitations. We developed an alternative approach, enzyme-catalyzed proximity labeling, for the high-resolution spatial mapping of subcellular proteomes and transcriptomes in living cells. I will describe the development of this approach, which includes enzyme directed evolution, and its application to some new mitochondrial biology.

In the second part of the talk, I will describe synthetic protease-based optogenetic circuits that convert transient molecular events into stable cellular signals. I will give an example of how these tools can be used to access and study specific neuronal subpopulations that are activated during particular animal behaviors. 

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