Ronald Raines

Department of Chemistry
Massachusetts Institute of Technology
Thursday, May. 5, 2022
Taylor Auditorium, Frick Chemistry Laboratory
Ralph Kleiner
Add to Calendar2022-05-05 16:30:002022-05-05 16:30:00Ralph KleinerTaylor Auditorium, Frick Chemistry Laboratory 15YYYY-MM-DD

Ghost Proteins

The lipid bilayer that encases human cells has evolved to keep the outside out, and the inside in. This barrier is not, however, impenetrable. Some small molecules, including many drugs, can burrow through and manifest therapeutic activities. Others can be “cloaked” to endow membrane permeability, and then uncloaked inside cells. We have learned how to beneficially cloak proteins, which are typically 100-fold larger than small-molecule drugs. Specifically, the use of tuned diazo compounds to convert protein carboxyl groups into esters in water enables a protein to traverse the lipid bilayer. The nascent esters are substrates for endogenous esterases that regenerate native proteins within cells. The ability to deliver native proteins directly into cells opens a new frontier for molecular medicine.

Research Areas